Journal: Journal of Tissue Engineering

Article Title: Developing human upper, lower, and deep lung airway models: Combining different scaffolds and developing complex co-cultures

doi: 10.1177/20417314241299076

Figure Lengend Snippet: Scanning electron microscope (SEM) images of the electrospun polymeric scaffold structures, depicting the difference in the porous structures generated through randomized fiber alignment as well as the variation seen in the fiber structure. (b, d, f) are the magnified images of the respective polymer mixture membranes in the a, c, and e, respectively.

Article Snippet: Meanwhile standard electrospun polymer membrane (50–60 μm thick), which possesses a considerably low modulus of elasticity, offered higher flexibility and supported co-cultures of primary alveolar epithelial (huAEC) and endothelial cells (hEC) in concert with lung biopsy-derived fibroblasts which enhanced maturation of the tissue model. As published, designing human alveolar in vitro models require thin scaffold to mimic the required ultra-thin ECM, in addition to assuring right balanced AT1/AT2 ratio for biomimetic representation.

Techniques: Microscopy, Generated, Polymer

Journal: BMC Nephrology

Article Title: Dissolved molecular hydrogen (H 2 ) in Peritoneal Dialysis (PD) solutions preserves mesothelial cells and peritoneal membrane integrity

doi: 10.1186/s12882-017-0741-0

Figure Lengend Snippet: Procedure for producing H 2 -dissolved peritoneal dialysis solution by the bathing method. a Time-course of changes in dissolved H 2 levels in electrolyzed water (*) and the solution in the PD bag (#). b Time-course of changes in dissolved H 2 levels in the solution in the PD bag when placed in air. Water was electrolyzed by Nafion (Synthetic polymer membrane; DuPont, Wilmington, DE) to generate highly dissolved H 2 water, with the concentration of H 2 exceeding 1.5 ppm. Then, the PD bag was bathed in the water to allow H 2 to shift into the PD solution by diffusion ( a ). Dissolved hydrogen is lost rapidly after loading once it is exposed to room air ( b ). Thus, the H 2 -dissolved PD solution was subjected to experiments within 1 h of 12-h bathing, to ensure a high level of H 2 in the PD solution (>400 ppm)

Article Snippet: Water was electrolyzed by Nafion (Synthetic polymer membrane; DuPont, Wilmington, DE) to generate highly dissolved H 2 water, with the concentration of H 2 exceeding 1.5 ppm.

Techniques: Polymer, Membrane, Concentration Assay, Diffusion-based Assay